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Towards clinically meaningful expansion of human HSCs
Maria Florencia Tellechea , Jalila Chagraoui , Sandra Cohen , Guy SauvageauInstitute for Research in Immunology and Cancer, University of Montreal, Montreal, QC, Canada
Improved strategies for ex vivo expansion of human hematopoietic stem cells (HSCs) are necessary to supply the large demand of cells required for certain clinical applications such as gene therapy/editing and cord blood (CB) transplantation. In a recent paper published in Nature, Sakurai et al. reported that the combination of three small molecules, namely a phosphoinositide 3-kinase activator peptide, a thrombopoietin receptor agonist and the pyrimidoindole derivative UM171, was sufficient to stimulate the expansion of umbilical CB HSCs, providing the first chemically defined cytokine-free expansion medium for human HSCs.