Volume 33, No 7, Jul 2023
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 33 Issue 7, July 2023: 493-494
A sticky solution to protein-selective sugar installation
Matthew R. Pratt*Department of Chemistry, University of Southern California, Los Angeles, CA, USA
O-GlcNAc is a widespread form of intracellular glycosylation that is added to thousands of substrates by one enzyme, O-GlcNAc transferase (OGT), and removed by one other enzyme, O-GlcNAcase (OGA); the generality of this biochemistry has made it extremely challenging to alter the levels of O-GlcNAc on selected proteins to investigate the consequences of specific modifications. In a recent Cell paper, Zhu et al. develop an RNA aptamer-based strategy to localize endogenous OGT to endogenous proteins of interest, resulting in increased O-GlcNAc modification.